| 550 |
QP67IE |
67I70E |
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View
Click on a Luminex® HLA allele that shares the eplet QP67IE to view it at pHLA3D
DPB1*01:01,
DPB1*02:01,
DPB1*04:01,
DPB1*04:02,
DPB1*05:01,
DPB1*09:01,
DPB1*10:01,
DPB1*13:01,
DPB1*17:01,
DPB1*18:01,
DPB1*19:01,
DPB1*23:01,
DQB1*04:01,
DQB1*04:02,
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DPB1*01:01, DPB1*02:01, DPB1*04:01, DPB1*04:02, DPB1*05:01, DPB1*09:01, DPB1*10:01, DPB1*13:01, DPB1*17:01, DPB1*18:01, DPB1*19:01, DPB1*23:01, DQB1*04:01, DQB1*04:02
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View (15)
DPB1*01:01, DPB1*02:01, DPB1*04:01, DPB1*04:02, DPB1*05:01, DPB1*09:01, DPB1*10:01, DPB1*13:01, DPB1*16:01, DPB1*17:01, DPB1*18:01, DPB1*19:01, DPB1*23:01, DQB1*04:01, DQB1*04:02
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| 551 |
QP77RV |
77R78V |
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View
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View
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DPB1*01:01, DPB1*03:01, DPB1*09:01, DPB1*10:01, DPB1*14:01, DQB1*02:01, DQB1*02:02, DQB1*05:01, DQB1*05:03
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View (9)
DPB1*01:01, DPB1*03:01, DPB1*09:01, DPB1*10:01, DPB1*14:01, DQB1*02:01, DQB1*02:02, DQB1*05:01, DQB1*05:03
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| 552 |
RP37FV |
37F38V |
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View
Click on a Luminex® HLA allele that shares the eplet RP37FV to view it at pHLA3D
DPB1*02:01,
DPB1*03:01,
DPB1*04:02,
DPB1*06:01,
DPB1*09:01,
DPB1*10:01,
DPB1*14:01,
DPB1*17:01,
DPB1*18:01,
DPB1*19:01,
DPB1*20:01,
DPB1*23:01,
DRB1*07:01,
DRB1*14:01,
DRB1*14:04,
DRB1*14:54,
DRB3*03:01,
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DPB1*02:01, DPB1*03:01, DPB1*04:02, DPB1*06:01, DPB1*09:01, DPB1*10:01, DPB1*14:01, DPB1*17:01, DPB1*18:01, DPB1*19:01, DPB1*20:01, DPB1*23:01, DRB1*07:01, DRB1*14:01, DRB1*14:04, DRB1*14:54, DRB3*03:01
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View (18)
DPB1*02:01, DPB1*03:01, DPB1*04:02, DPB1*06:01, DPB1*09:01, DPB1*10:01, DPB1*14:01, DPB1*16:01, DPB1*17:01, DPB1*18:01, DPB1*19:01, DPB1*20:01, DPB1*23:01, DRB1*07:01, DRB1*14:01, DRB1*14:04, DRB1*14:54, DRB3*03:01
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| 553 |
RP58E |
57D58E |
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View
Click on a Luminex® HLA allele that shares the eplet RP58E to view it at pHLA3D
DPB1*02:01,
DPB1*03:01,
DPB1*04:02,
DPB1*06:01,
DPB1*09:01,
DPB1*10:01,
DPB1*14:01,
DPB1*17:01,
DPB1*18:01,
DPB1*20:01,
DPB1*28:01,
DRB1*11:01,
DRB1*11:03,
DRB1*11:04,
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Yes
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rp58E on DR11 and HLA-DPB1*02, *03, *04:02, *06, *09, *10, *14, *16 *17, *18, *20, and *28
This eplet was previously named 57DEDP in the DRB registry database and 55EDR11 in the DPB registry database.
Note: 58E is represented by 57D-58E on DR11 which is equivalent to 55D-56E on DPB alleles
Three post-pregnancy sera from Stefan Schaub (Basel) are shown below. Please note, the antibody reactivity is not affected whether amino acid "D" or "E" is present in position 59 (DRB) or 57 (DPB).
Bodmer JG, Bodmer W, Heyes J. 1987. Identification of HLA-DP polymorphism with DP alpha and DP beta probes and monoclonal antibodies: Correlation with primed lymphocyte typing. Proc. Nat. Acad. Sci. USA 84: 4596-600. Mouse I-LR1 mAb recognize an epitope on DR11 and 56E-carrying DPB molecules that share 56DEE or DED.
Pistillo, M. P., O. Mazzoleni, et al. (1991). "Production of two human hybridomas secreting antibodies to HLA-DRw11 and--DRw8+w12 specificities." Human Immunology 31(2): 86-93.The DR11-induced human mAb MP4 reacts with HLA-DR11 and 56E-carrying DPB alleles in cytofluorometry and CDC assays.
Marshall, W. H., S. Drover, et al. (1998). "HLA-DP epitope typing using monoclonal antibodies." Human Immunology. 59(3): 189-197. The DPB*04:02/DPA1*01-induced mouse mAb NFLD.M58 reacted with all 56E-carrying DPB1 alleles and the 58E-carrying DR11. In both CDC and Flow cytometry r value = 1.0. mAb NFLD.M73 had a similar reactivity pattern.
Inoko, H., J. G. Bodmer, et al. (1991). Joint report on the transfectant/monoclonal antibody component. HLA 1991 Proceedings of the Eleventh International Histocompatibility Workshop and Conference. K. Tsuji, M. Aizawa and T. Sasazuki. Oxford, UK, Oxford University Press. I: 919-930. Same specificity patterns for NFLD.M58 and NFLD.M64.
Billen, E. V. A., M. H. L. Christiaans, et al. (2010). "HLA-DP antibodies before and after renal transplantation." Tissue Antigens 75(3): 278-285. A transplant recipient (DPB1*01:01, 04:01; donor: DPB1*04:02,*06:01) and a pre-transplant patient (DB1*04:01,*04:01) sensitized by pregnancy (husband (DPB1*02:01,*17:01) had antibodies reacting with 56E (DED/DEE) Not clear if there was DR11 reactivity.
Klohe E, Pistillo MP, Ferrara GB, Goeken NE, Greazel NS, Karr RW. 1992. Critical role of HLA-DR beta 1 residue 58 in multiple polymorphic epitopes recognized by xenogeneic and allogeneic antibodies. Human Immunology. 35: 18-28. Mutated DR11 with E58A or E58D substitutions lost reactivity with MP4 and I-LR1 but D57A had no effect. Moreover, mutated DRB1, DRB3 and DRB5 chains with A58E substitutions became reactive. These findings suggest that 58E plays a dominant role in this epitope but may require another configuration shared between DRB1, DRB3, DRB5 and DPB. Mutated DR11 chains with E9W, Y10Q, S11L, T12K, S13F, D28E, Y30C, Y37S, F67L, D70Q, D70R, R71E, A74Q, A74E and T77N continued to react with MP4 and I-LR2. It was suggested that the additional configuration may include residues 41Q, 44L and/or 48R.
Yu, W. Y., R. Watts, et al. (1990). "Identification of amino acids in HLA-DPw4 and -DR51 chains that are involved in antibody binding epitopes using site-directed mutagenesis and DNA-mediated gene transfer." Human Immunology. 27(2): 122-135. The E56A substitution of mutated DPB1*04:02 and the E58A substitution of mutated DRB1*11 abolished binding with I-LR1.
Diaz, G., M. Amicosante, et al. (2003). "Functional analysis of HLA-DP polymorphism: a crucial role for DPB residues 9, 11, 35, 55, 56, 69 and 84-87 in T cell allorecognition and peptide binding." International Immunology 15: 565-576. The E56A substitution on mutated DRB1*02:01 abolished reactivity with mAb ILR1.
Vaidya, S., B. Hilson, et al. (2007). "DP reactive antibody in a zero mismatch renal transplant pair." Human Immunology 68(12): 947-949. A zero-ABCDRDDQ but a DPB1*06:01 mismatched renal transplant led to antibodies that reacted with 56E-carrying DPB alleles plus the 58E-carrying DR11.
Cano, P. and M. Fernandez-Vina (2009). "Two sequence dimorphisms of DPB1 define the immunodominant serologic epitopes of HLA-DP." Human Immunology 70(10): 836-843. Epitopes on HLA-DP molecules were identified with alloantibodies from clinical serum samples and tested with single antigens on a Luminex platform. Residue 56E defines epitope P-002 and was identified by 9 sera. Interlocus DRB1*11/DPB1 reactivity is associated with P-002.
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DPB1*02:01, DPB1*03:01, DPB1*04:02, DPB1*06:01, DPB1*09:01, DPB1*10:01, DPB1*14:01, DPB1*17:01, DPB1*18:01, DPB1*20:01, DPB1*28:01, DRB1*11:01, DRB1*11:03, DRB1*11:04
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View (109)
DPB1*02:01, DPB1*03:01, DPB1*04:02, DPB1*06:01, DPB1*08:01, DPB1*09:01, DPB1*10:01, DPB1*14:01, DPB1*16:01, DPB1*17:01, DPB1*18:01, DPB1*20:01, DPB1*25:01, DPB1*28:01, DPB1*29:01, DPB1*32:01, DPB1*35:01, DPB1*37:01, DPB1*41:01, DPB1*44:01, DPB1*45:01, DPB1*46:01, DPB1*48:01, DPB1*49:01, DPB1*50:01, DPB1*51:01, DPB1*53:01, DPB1*57:01, DPB1*59:01, DPB1*60:01, DPB1*68:01, DPB1*69:01, DPB1*70:01, DPB1*73:01, DPB1*75:01, DPB1*76:01, DPB1*77:01, DPB1*78:01, DPB1*79:01, DPB1*80:01, DPB1*81:01, DPB1*82:01, DPB1*83:01, DPB1*86:01, DPB1*88:01, DPB1*91:01, DPB1*92:01, DPB1*93:01, DPB1*94:01, DPB1*98:01, DRB1*03:08, DRB1*04:15, DRB1*11:01, DRB1*11:02, DRB1*11:03, DRB1*11:04, DRB1*11:05, DRB1*11:06, DRB1*11:07, DRB1*11:08, DRB1*11:09, DRB1*11:10, DRB1*11:11, DRB1*11:12, DRB1*11:13, DRB1*11:14, DRB1*11:15, DRB1*11:16, DRB1*11:17, DRB1*11:18, DRB1*11:19, DRB1*11:20, DRB1*11:21, DRB1*11:22, DRB1*11:23, DRB1*11:24, DRB1*11:25, DRB1*11:26, DRB1*11:27, DRB1*11:28, DRB1*11:29, DRB1*11:30, DRB1*11:31, DRB1*11:32, DRB1*11:33, DRB1*11:34, DRB1*11:35, DRB1*11:36, DRB1*11:37, DRB1*11:38, DRB1*11:39, DRB1*11:40, DRB1*11:41, DRB1*11:42, DRB1*11:43, DRB1*11:44, DRB1*11:45, DRB1*11:46, DRB1*11:47, DRB1*11:48, DRB1*11:49, DRB1*11:50, DRB1*11:51, DRB1*11:52, DRB1*11:53, DRB1*12:04, DRB1*14:11, DRB3*02:18, DRB5*01:13
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| 554 |
RP58EE |
57D58E59E |
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View
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Yes
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rp58EE on DR11 and DPB1*02, *04:02, *10, *16, *18 and *28
This eplet was previously named 58EEDP in the DRB registry database and 56EEDR11 in the DPB registry database.
Note: the 57D58E59E configuration on DR11 is equivalent to the 55D56E57E configuration on DPB.
Marshall, W. H., S. Drover, et al. (1998). "HLA-DP epitope typing using monoclonal antibodies." Human Immunology. 59(3): 189-197. DPB1*04:02-induced mouse mAb NFLD.M106 reacts with HLA-DPB1 alleles that share residues DEE at positions 55-57 in CDC, flow cytometry and ELISA assays. Antibody also reacts with DR11 alleles which share residues DE at positions 57-58. CDC and Flow cytometry correlation: r value = 1.0; ELISA correlation: r value = 1.0
Inoko, H., J. G. Bodmer, et al. (1991). Joint report on the transfectant/ monoclonal antibody component. HLA 1991 Proceedings of the Eleventh International Histocompatibility Workshop and Conference. K. Tsuji, M. Aizawa and T. Sasazuki. Oxford, UK, Oxford University Press. I: 919-930. The DPB*09:01/DPA1*02-induced NFLD.M60 reacted with all 57E (DEE) carrying DPB1 alleles plus DR11 which has DEE.
Tongio MM, Van Den Berg Loonan E, Bignon J, et al. 1997. “HLA-DP detected by serology” in HLA - Genetic Diversity of HLA – Functional and Medical Implications, vol. II – Proceedings of the Twelfth International Histocompatibility Workshop and Conference. D. Charron (Ed), EDK, Paris France. p. 135-138. Mouse mAb M106 positive with EBV cell lines and peripheral B lymphocytes that have HLA-DPB1alleles that share residues 55D-56E-57E (r = 1.0). Antibody reacts also with HLA-DR11 which shares residues DEE at positions 57-59.
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DPB1*02:01, DPB1*04:02, DPB1*10:01, DPB1*18:01, DPB1*28:01, DRB1*11:01, DRB1*11:03, DRB1*11:04
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View (83)
DPB1*02:01, DPB1*04:02, DPB1*08:01, DPB1*10:01, DPB1*16:01, DPB1*18:01, DPB1*25:01, DPB1*28:01, DPB1*37:01, DPB1*41:01, DPB1*45:01, DPB1*48:01, DPB1*49:01, DPB1*51:01, DPB1*53:01, DPB1*59:01, DPB1*60:01, DPB1*68:01, DPB1*73:01, DPB1*75:01, DPB1*77:01, DPB1*79:01, DPB1*81:01, DPB1*82:01, DPB1*83:01, DPB1*93:01, DPB1*94:01, DRB1*03:08, DRB1*04:15, DRB1*11:01, DRB1*11:02, DRB1*11:03, DRB1*11:04, DRB1*11:05, DRB1*11:06, DRB1*11:07, DRB1*11:08, DRB1*11:09, DRB1*11:10, DRB1*11:11, DRB1*11:12, DRB1*11:13, DRB1*11:14, DRB1*11:15, DRB1*11:16, DRB1*11:17, DRB1*11:18, DRB1*11:19, DRB1*11:20, DRB1*11:21, DRB1*11:22, DRB1*11:23, DRB1*11:24, DRB1*11:25, DRB1*11:26, DRB1*11:27, DRB1*11:28, DRB1*11:29, DRB1*11:30, DRB1*11:31, DRB1*11:32, DRB1*11:34, DRB1*11:36, DRB1*11:37, DRB1*11:39, DRB1*11:40, DRB1*11:41, DRB1*11:42, DRB1*11:43, DRB1*11:44, DRB1*11:45, DRB1*11:46, DRB1*11:47, DRB1*11:48, DRB1*11:49, DRB1*11:50, DRB1*11:51, DRB1*11:52, DRB1*11:53, DRB1*12:04, DRB1*14:11, DRB3*02:18, DRB5*01:13
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| 555 |
RP67IE |
67I71E |
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View
Click on a Luminex® HLA allele that shares the eplet RP67IE to view it at pHLA3D
DPB1*02:01,
DPB1*02:02,
DPB1*09:01,
DPB1*10:01,
DPB1*13:01,
DPB1*17:01,
DPB1*19:01,
DPB1*30:01,
DRB1*01:03,
DRB1*04:02,
DRB1*13:01,
DRB1*13:02,
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Yes
rp67IE on DRB1*01:03 and DPB1*02, *09, *10, *13, *16 and *17
CJ Callender, M Fernandez-Vina, MS Leffell, AA Zachary: Frequency of HLA-DP-specific antibodies and a possible new cross-reacting group. Human Immunology 73:175-179, 2012
This report describes a strong cross-reactivity between DPB and DRB1*01:03 in the absence of DR1 or DR11 reactivity. Sequence alignments were performed and a possible new cross-reactivity between DRB1*01:03 and DP2, DP9, DP10, DP13, DP16, and DP17 was defined. Two additional sera confirmed this cross-reactivity. No antibody reactivity with HLA panels is shown. This report describes an epitope described by 65I+67E on DP and 67I+69E on DR but we prefer 65I+69E and 67I+71E. It seems that the 67IE-carrying DRB1*11:02 and DRB1*13:01 were weakly reactive and;they have a nearby distinct 26D residue below 67IE.
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DPB1*02:01, DPB1*02:02, DPB1*09:01, DPB1*10:01, DPB1*13:01, DPB1*17:01, DPB1*19:01, DPB1*30:01, DRB1*01:03, DRB1*04:02, DRB1*13:01, DRB1*13:02
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View (67)
DPB1*02:01, DPB1*02:02, DPB1*08:01, DPB1*09:01, DPB1*10:01, DPB1*13:01, DPB1*16:01, DPB1*17:01, DPB1*19:01, DPB1*21:01, DPB1*22:01, DPB1*30:01, DPB1*32:01, DPB1*33:01, DPB1*37:01, DPB1*46:01, DPB1*47:01, DPB1*48:01, DPB1*54:01, DPB1*55:01, DPB1*58:01, DPB1*71:01, DPB1*81:01, DPB1*86:01, DPB1*88:01, DPB1*93:01, DRB1*01:03, DRB1*04:02, DRB1*04:14, DRB1*11:02, DRB1*11:14, DRB1*11:16, DRB1*11:20, DRB1*11:21, DRB1*11:48, DRB1*13:01, DRB1*13:02, DRB1*13:04, DRB1*13:08, DRB1*13:15, DRB1*13:16, DRB1*13:17, DRB1*13:19, DRB1*13:22, DRB1*13:23, DRB1*13:27, DRB1*13:28, DRB1*13:31, DRB1*13:32, DRB1*13:34, DRB1*13:35, DRB1*13:36, DRB1*13:38, DRB1*13:39, DRB1*13:40, DRB1*13:41, DRB1*13:45, DRB1*13:48, DRB1*13:51, DRB1*13:52, DRB1*13:53, DRB1*13:57, DRB1*13:59, DRB1*13:61, DRB1*13:64, DRB1*14:16, DRB1*15:10
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| 556 |
RP67LK |
67L71K |
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View
Click on a Luminex® HLA allele that shares the eplet RP67LK to view it at pHLA3D
DPB1*03:01,
DPB1*14:01,
DPB1*20:01,
DPB1*28:01,
DRB1*03:01,
DRB1*03:02,
DRB1*03:03,
DRB1*04:01,
DRB3*01:01,
DRB3*02:01,
DRB3*02:02,
DRB3*03:01,
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DPB1*03:01, DPB1*14:01, DPB1*20:01, DPB1*28:01, DRB1*03:01, DRB1*03:02, DRB1*03:03, DRB1*04:01, DRB3*01:01, DRB3*02:01, DRB3*02:02, DRB3*03:01
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View (12)
DPB1*03:01, DPB1*14:01, DPB1*20:01, DPB1*28:01, DRB1*03:01, DRB1*03:02, DRB1*03:03, DRB1*04:01, DRB3*01:01, DRB3*02:01, DRB3*02:02, DRB3*03:01
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| 557 |
RP67LR |
67L71R |
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View
Click on a Luminex® HLA allele that shares the eplet RP67LR to view it at pHLA3D
DPB1*11:01,
DPB1*15:01,
DRB1*01:01,
DRB1*01:02,
DRB1*04:03,
DRB1*04:04,
DRB1*04:05,
DRB1*10:01,
DRB1*14:01,
DRB1*14:02,
DRB1*14:03,
DRB1*14:04,
DRB1*14:54,
DRB1*16:02,
DRB4*01:01,
DRB4*01:03,
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DPB1*11:01, DPB1*15:01, DRB1*01:01, DRB1*01:02, DRB1*04:03, DRB1*04:04, DRB1*04:05, DRB1*10:01, DRB1*14:01, DRB1*14:02, DRB1*14:03, DRB1*14:04, DRB1*14:54, DRB1*16:02, DRB4*01:01, DRB4*01:03
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View (16)
DPB1*11:01, DPB1*15:01, DRB1*01:01, DRB1*01:02, DRB1*04:03, DRB1*04:04, DRB1*04:05, DRB1*10:01, DRB1*14:01, DRB1*14:02, DRB1*14:03, DRB1*14:04, DRB1*14:54, DRB1*16:02, DRB4*01:01, DRB4*01:03
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| 558 |
RQ140TV |
140T142V |
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View
Click on a Luminex® HLA allele that shares the eplet RQ140TV to view it at pHLA3D
DQB1*03:01,
DQB1*03:02,
DQB1*03:03,
DQB1*04:01,
DQB1*04:02,
DRB1*03:01,
DRB1*03:02,
DRB1*03:03,
DRB1*04:01,
DRB1*04:02,
DRB1*04:03,
DRB1*04:04,
DRB1*04:05,
DRB1*08:01,
DRB1*10:01,
DRB1*11:01,
DRB1*11:03,
DRB1*11:04,
DRB1*12:01,
DRB1*12:02,
DRB1*13:01,
DRB1*13:02,
DRB1*13:03,
DRB1*13:05,
DRB1*14:01,
DRB1*14:02,
DRB1*14:03,
DRB1*14:04,
DRB1*14:54,
DRB3*03:01,
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DQB1*03:01, DQB1*03:02, DQB1*03:03, DQB1*04:01, DQB1*04:02, DRB1*03:01, DRB1*03:02, DRB1*03:03, DRB1*04:01, DRB1*04:02, DRB1*04:03, DRB1*04:04, DRB1*04:05, DRB1*08:01, DRB1*10:01, DRB1*11:01, DRB1*11:03, DRB1*11:04, DRB1*12:01, DRB1*12:02, DRB1*13:01, DRB1*13:02, DRB1*13:03, DRB1*13:05, DRB1*14:01, DRB1*14:02, DRB1*14:03, DRB1*14:04, DRB1*14:54, DRB3*03:01
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View (30)
DQB1*03:01, DQB1*03:02, DQB1*03:03, DQB1*04:01, DQB1*04:02, DRB1*03:01, DRB1*03:02, DRB1*03:03, DRB1*04:01, DRB1*04:02, DRB1*04:03, DRB1*04:04, DRB1*04:05, DRB1*08:01, DRB1*10:01, DRB1*11:01, DRB1*11:03, DRB1*11:04, DRB1*12:01, DRB1*12:02, DRB1*13:01, DRB1*13:02, DRB1*13:03, DRB1*13:05, DRB1*14:01, DRB1*14:02, DRB1*14:03, DRB1*14:04, DRB1*14:54, DRB3*03:01
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| 559 |
RQ26Y |
26Y |
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View
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DQB1*03:01, DQB1*06:01, DRB1*03:01, DRB1*09:01, DRB1*09:02, DRB3*01:01
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View (6)
DQB1*03:01, DQB1*06:01, DRB1*03:01, DRB1*09:01, DRB1*09:02, DRB3*01:01
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| 560 |
RQ37YV |
37Y38V |
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View
Click on a Luminex® HLA allele that shares the eplet RQ37YV to view it at pHLA3D
DQB1*05:01,
DQB1*05:03,
DRB1*04:01,
DRB1*04:02,
DRB1*04:03,
DRB1*04:04,
DRB1*04:05,
DRB1*08:01,
DRB1*11:01,
DRB1*11:03,
DRB1*11:04,
DRB1*13:03,
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View
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DQB1*05:01, DQB1*05:03, DRB1*04:01, DRB1*04:02, DRB1*04:03, DRB1*04:04, DRB1*04:05, DRB1*08:01, DRB1*11:01, DRB1*11:03, DRB1*11:04, DRB1*13:03
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View (12)
DQB1*05:01, DQB1*05:03, DRB1*04:01, DRB1*04:02, DRB1*04:03, DRB1*04:04, DRB1*04:05, DRB1*08:01, DRB1*11:01, DRB1*11:03, DRB1*11:04, DRB1*13:03
|
| 561 |
RQ57S |
57S |
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View
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DQB1*05:02, DRB1*04:05, DRB1*04:10, DRB1*04:11, DRB1*08:01, DRB1*08:03, DRB1*13:03
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View (47)
DQB1*05:02, DQB1*05:04, DQB1*06:10, DRB1*03:12, DRB1*04:05, DRB1*04:09, DRB1*04:10, DRB1*04:11, DRB1*04:12, DRB1*04:17, DRB1*04:24, DRB1*04:28, DRB1*04:29, DRB1*04:30, DRB1*04:45, DRB1*04:48, DRB1*08:01, DRB1*08:03, DRB1*08:05, DRB1*08:06, DRB1*08:10, DRB1*08:12, DRB1*08:14, DRB1*08:16, DRB1*08:17, DRB1*08:18, DRB1*08:22, DRB1*08:23, DRB1*08:26, DRB1*08:27, DRB1*08:29, DRB1*13:03, DRB1*13:04, DRB1*13:12, DRB1*13:13, DRB1*13:21, DRB1*13:30, DRB1*13:32, DRB1*13:33, DRB1*13:38, DRB1*13:48, DRB1*13:49, DRB1*13:55, DRB1*13:58, DRB1*14:13, DRB1*15:12, DRB3*02:08
|
| 562 |
RQ57V |
57V |
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View
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View
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DQB1*05:01, DQB1*06:04, DQB1*06:09, DRB1*07:01, DRB1*09:01, DRB1*12:01, DRB1*12:02, DRB3*01:01, DRB3*03:01
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View (9)
DQB1*05:01, DQB1*06:04, DQB1*06:09, DRB1*07:01, DRB1*09:01, DRB1*12:01, DRB1*12:02, DRB3*01:01, DRB3*03:01
|
| 563 |
RQ70RK/R |
70R71K/R |
|
View
Click on a Luminex® HLA allele that shares the eplet RQ70RK/R to view it at pHLA3D
DQB1*02:01,
DQB1*02:02,
DRB1*09:01,
DRB1*09:02,
DRB1*10:01,
DRB1*14:01,
DRB1*14:04,
DRB1*14:05,
DRB1*14:54,
DRB4*01:01,
DRB4*01:03,
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Yes
|
rq70RK/R on DQ2, DR9, DR10, DRB1*14:01 and possibly DR53
S Drover, J, Gamber, C Qi and WH Marshall: Identification of a shared determinant on DQB1*0201 and DRB1*09, DRB1*I001 and DRB1*1401 molecules by a monoclonal antibody, ASHI Meeting Abstract B1.2.9 Hum Immunol 34: 11, 1992.
Mouse mAb NFLD.M71 recognizes a polymorphic determinant on DQw2 molecules and also binds
to a set of DR molecules which share a short patch of homology around residue 70 with DQB1*0201 molecules. This mAb was derived from a fusion using a Balb/C mouse immunized with class II molecules from a homozygous B cell line (10th IHW 9022). Reactivity patterns obtained from binding assays (ELISA and FACS) using homozygous cells showed that NFLD.M71 binds DQB1*0201 chains as well as DRB1*1401, DR9 and DR10. From an amino acid sequence analysis, it appears that the structural determinant detected by NFLD.M71 is influenced by residues 70-74, RKRAA on
DQB1*0201 chains and RRRAA/RRRAE on DRB1*09, 1001, and 1401 chains.
Our residue analysis predicts that this mAb will also react with DRB4 alleles.
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DQB1*02:01, DQB1*02:02, DRB1*09:01, DRB1*09:02, DRB1*10:01, DRB1*14:01, DRB1*14:04, DRB1*14:05, DRB1*14:54, DRB4*01:01, DRB4*01:03
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View (42)
DQB1*02:01, DQB1*02:02, DQB1*02:03, DRB1*04:24, DRB1*09:01, DRB1*09:02, DRB1*10:01, DRB1*11:13, DRB1*11:17, DRB1*11:52, DRB1*14:01, DRB1*14:04, DRB1*14:05, DRB1*14:07, DRB1*14:08, DRB1*14:10, DRB1*14:11, DRB1*14:14, DRB1*14:18, DRB1*14:23, DRB1*14:26, DRB1*14:28, DRB1*14:31, DRB1*14:32, DRB1*14:34, DRB1*14:35, DRB1*14:36, DRB1*14:38, DRB1*14:39, DRB1*14:42, DRB1*14:43, DRB1*14:44, DRB1*14:45, DRB1*14:54, DRB4*01:01, DRB4*01:02, DRB4*01:03, DRB4*01:04, DRB4*01:05, DRB4*01:06, DRB4*01:07, DRB5*01:12
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| 564 |
RQ74AV |
74A75V |
|
View
Click on a Luminex® HLA allele that shares the eplet RQ74AV to view it at pHLA3D
DQB1*02:01,
DQB1*02:02,
DRB1*01:01,
DRB1*01:02,
DRB1*01:03,
DRB1*04:01,
DRB1*04:02,
DRB1*04:04,
DRB1*04:05,
DRB1*10:01,
DRB1*11:01,
DRB1*11:03,
DRB1*11:04,
DRB1*12:01,
DRB1*12:02,
DRB1*13:01,
DRB1*13:02,
DRB1*13:03,
DRB1*13:05,
DRB1*14:02,
DRB1*15:01,
DRB1*15:02,
DRB1*15:03,
DRB1*16:01,
DRB1*16:02,
DRB5*01:01,
DRB5*02:02,
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View
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DQB1*02:01, DQB1*02:02, DRB1*01:01, DRB1*01:02, DRB1*01:03, DRB1*04:01, DRB1*04:02, DRB1*04:04, DRB1*04:05, DRB1*10:01, DRB1*11:01, DRB1*11:03, DRB1*11:04, DRB1*12:01, DRB1*12:02, DRB1*13:01, DRB1*13:02, DRB1*13:03, DRB1*13:05, DRB1*14:02, DRB1*15:01, DRB1*15:02, DRB1*15:03, DRB1*16:01, DRB1*16:02, DRB5*01:01, DRB5*02:02
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View (27)
DQB1*02:01, DQB1*02:02, DRB1*01:01, DRB1*01:02, DRB1*01:03, DRB1*04:01, DRB1*04:02, DRB1*04:04, DRB1*04:05, DRB1*10:01, DRB1*11:01, DRB1*11:03, DRB1*11:04, DRB1*12:01, DRB1*12:02, DRB1*13:01, DRB1*13:02, DRB1*13:03, DRB1*13:05, DRB1*14:02, DRB1*15:01, DRB1*15:02, DRB1*15:03, DRB1*16:01, DRB1*16:02, DRB5*01:01, DRB5*02:02
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| 565 |
RQ75VT |
75V77T |
|
View
Click on a Luminex® HLA allele that shares the eplet RQ75VT to view it at pHLA3D
DQB1*04:01,
DQB1*04:02,
DRB1*01:01,
DRB1*01:02,
DRB1*01:03,
DRB1*04:01,
DRB1*04:02,
DRB1*04:03,
DRB1*04:04,
DRB1*04:05,
DRB1*04:06,
DRB1*04:07,
DRB1*04:10,
DRB1*04:11,
DRB1*07:01,
DRB1*08:01,
DRB1*08:02,
DRB1*08:03,
DRB1*08:07,
DRB1*09:01,
DRB1*09:02,
DRB1*10:01,
DRB1*11:01,
DRB1*11:03,
DRB1*11:04,
DRB1*12:01,
DRB1*12:02,
DRB1*13:01,
DRB1*13:02,
DRB1*13:03,
DRB1*13:05,
DRB1*14:01,
DRB1*14:02,
DRB1*14:03,
DRB1*14:04,
DRB1*14:05,
DRB1*14:06,
DRB1*14:54,
DRB1*15:01,
DRB1*15:02,
DRB1*15:03,
DRB1*16:01,
DRB1*16:02,
DRB4*01:01,
DRB4*01:03,
DRB5*01:01,
DRB5*01:02,
DRB5*02:02,
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Yes
rq75VT on several DRB alleles and DQ4
The IgM human mAb JOK1H7 originating in Arend Mulder’s Laboratory at Leiden University Medical Center reacts with 75VT-carrying DRB alleles and additionally, with DQ4 heterodimers which also have 75VT. All other DQ dimers were essentially negative. This antibody originated from a woman who types as DRB1*03:01, *13:01, DRB3*01:01, DQB1*02:01, *06:03 and she was HLA sensitized during pregnancy. Although the HLA type of this child is unknown, her husband types as DRB1*04:02, *11:01, DRB3*02:02, DRB4*01:03, DQB1*03:02. This mAb reacted with husband’s DRB4*01:03 (MFI=7349) and DRB1*11:01 (MFI=3664).
The MFI data with the entire DR and DQ panel are shown below. None of the DP dimers reacted with this mAb (data not shown). Positive reactions with DRB alleles were limited to 75VT-carrying DRB alleles except those that have the combination of nearby residues 67I and 70D. This suggests that the epitope defined by 75VT includes residues in sequence positions 67 and 70; the presence of 67I+70D abolishes the reactivity with this antibody. We noted significantly lower MFI values (3551 versus 11792, p<0.00001) for the seven 75VT-carrying DRB alleles that have 70D together with 67F or 67L. This means that the presence of 70D has also an inhibitory effect. In contrast, the four DRB alleles which have 67I+70Q gave similarly high MFI values and the remaining 75VT-carrying DRB alleles also reacted well regardless of the presence of 67L and 67F together with 70R and 70Q.
All four DQ4 heterodimers were distinctly reactive; their average MFI values was 4733. They carry 75VT together with 67I+70E. Since these data were obtained with a mAb which by definition, must have just one specificity, these findings indicate the antibody verification of a 75VT-related epitope shared between DRB and DQB. The locations of relevant residues are depicted in the molecular models below.
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View
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DQB1*04:01, DQB1*04:02, DRB1*01:01, DRB1*01:02, DRB1*01:03, DRB1*04:01, DRB1*04:02, DRB1*04:03, DRB1*04:04, DRB1*04:05, DRB1*04:06, DRB1*04:07, DRB1*04:10, DRB1*04:11, DRB1*07:01, DRB1*08:01, DRB1*08:02, DRB1*08:03, DRB1*08:07, DRB1*09:01, DRB1*09:02, DRB1*10:01, DRB1*11:01, DRB1*11:03, DRB1*11:04, DRB1*12:01, DRB1*12:02, DRB1*13:01, DRB1*13:02, DRB1*13:03, DRB1*13:05, DRB1*14:01, DRB1*14:02, DRB1*14:03, DRB1*14:04, DRB1*14:05, DRB1*14:06, DRB1*14:54, DRB1*15:01, DRB1*15:02, DRB1*15:03, DRB1*16:01, DRB1*16:02, DRB4*01:01, DRB4*01:03, DRB5*01:01, DRB5*01:02, DRB5*02:02
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View (314)
DQB1*03:06, DQB1*04:01, DQB1*04:02, DRB1*01:01, DRB1*01:02, DRB1*01:03, DRB1*01:05, DRB1*01:06, DRB1*01:07, DRB1*01:08, DRB1*01:09, DRB1*01:10, DRB1*03:14, DRB1*03:15, DRB1*04:01, DRB1*04:02, DRB1*04:03, DRB1*04:04, DRB1*04:05, DRB1*04:06, DRB1*04:07, DRB1*04:08, DRB1*04:09, DRB1*04:10, DRB1*04:11, DRB1*04:12, DRB1*04:13, DRB1*04:14, DRB1*04:15, DRB1*04:16, DRB1*04:17, DRB1*04:18, DRB1*04:19, DRB1*04:20, DRB1*04:21, DRB1*04:23, DRB1*04:24, DRB1*04:25, DRB1*04:26, DRB1*04:27, DRB1*04:28, DRB1*04:29, DRB1*04:30, DRB1*04:31, DRB1*04:32, DRB1*04:33, DRB1*04:34, DRB1*04:35, DRB1*04:36, DRB1*04:37, DRB1*04:38, DRB1*04:39, DRB1*04:40, DRB1*04:41, DRB1*04:42, DRB1*04:43, DRB1*04:45, DRB1*04:46, DRB1*04:47, DRB1*04:48, DRB1*04:49, DRB1*04:50, DRB1*07:01, DRB1*07:03, DRB1*07:05, DRB1*07:06, DRB1*07:07, DRB1*07:08, DRB1*08:01, DRB1*08:02, DRB1*08:03, DRB1*08:04, DRB1*08:05, DRB1*08:06, DRB1*08:07, DRB1*08:08, DRB1*08:09, DRB1*08:10, DRB1*08:11, DRB1*08:12, DRB1*08:13, DRB1*08:14, DRB1*08:15, DRB1*08:16, DRB1*08:17, DRB1*08:18, DRB1*08:19, DRB1*08:20, DRB1*08:21, DRB1*08:22, DRB1*08:23, DRB1*08:24, DRB1*08:25, DRB1*08:26, DRB1*08:27, DRB1*08:28, DRB1*08:29, DRB1*09:01, DRB1*09:02, DRB1*09:03, DRB1*10:01, DRB1*11:01, DRB1*11:02, DRB1*11:03, DRB1*11:04, DRB1*11:05, DRB1*11:06, DRB1*11:08, DRB1*11:09, DRB1*11:10, DRB1*11:11, DRB1*11:12, DRB1*11:13, DRB1*11:14, DRB1*11:15, DRB1*11:16, DRB1*11:17, DRB1*11:18, DRB1*11:19, DRB1*11:20, DRB1*11:21, DRB1*11:22, DRB1*11:23, DRB1*11:24, DRB1*11:25, DRB1*11:26, DRB1*11:28, DRB1*11:29, DRB1*11:30, DRB1*11:31, DRB1*11:32, DRB1*11:33, DRB1*11:34, DRB1*11:35, DRB1*11:36, DRB1*11:37, DRB1*11:38, DRB1*11:39, DRB1*11:40, DRB1*11:41, DRB1*11:42, DRB1*11:43, DRB1*11:44, DRB1*11:45, DRB1*11:46, DRB1*11:47, DRB1*11:48, DRB1*11:49, DRB1*11:50, DRB1*11:51, DRB1*11:52, DRB1*11:53, DRB1*12:01, DRB1*12:02, DRB1*12:03, DRB1*12:04, DRB1*12:05, DRB1*12:06, DRB1*12:07, DRB1*12:08, DRB1*12:09, DRB1*12:10, DRB1*13:01, DRB1*13:02, DRB1*13:03, DRB1*13:04, DRB1*13:05, DRB1*13:06, DRB1*13:07, DRB1*13:08, DRB1*13:09, DRB1*13:10, DRB1*13:11, DRB1*13:12, DRB1*13:13, DRB1*13:14, DRB1*13:15, DRB1*13:16, DRB1*13:17, DRB1*13:18, DRB1*13:19, DRB1*13:20, DRB1*13:21, DRB1*13:22, DRB1*13:23, DRB1*13:24, DRB1*13:25, DRB1*13:26, DRB1*13:27, DRB1*13:28, DRB1*13:29, DRB1*13:30, DRB1*13:31, DRB1*13:32, DRB1*13:34, DRB1*13:35, DRB1*13:36, DRB1*13:37, DRB1*13:38, DRB1*13:39, DRB1*13:40, DRB1*13:41, DRB1*13:42, DRB1*13:43, DRB1*13:44, DRB1*13:45, DRB1*13:46, DRB1*13:47, DRB1*13:48, DRB1*13:49, DRB1*13:50, DRB1*13:51, DRB1*13:52, DRB1*13:53, DRB1*13:54, DRB1*13:55, DRB1*13:56, DRB1*13:57, DRB1*13:58, DRB1*13:59, DRB1*13:60, DRB1*13:62, DRB1*13:63, DRB1*13:64, DRB1*14:01, DRB1*14:02, DRB1*14:03, DRB1*14:04, DRB1*14:05, DRB1*14:06, DRB1*14:07, DRB1*14:08, DRB1*14:09, DRB1*14:10, DRB1*14:11, DRB1*14:12, DRB1*14:13, DRB1*14:14, DRB1*14:15, DRB1*14:16, DRB1*14:17, DRB1*14:18, DRB1*14:19, DRB1*14:20, DRB1*14:21, DRB1*14:22, DRB1*14:23, DRB1*14:24, DRB1*14:25, DRB1*14:26, DRB1*14:27, DRB1*14:28, DRB1*14:29, DRB1*14:30, DRB1*14:31, DRB1*14:32, DRB1*14:33, DRB1*14:34, DRB1*14:35, DRB1*14:36, DRB1*14:37, DRB1*14:39, DRB1*14:40, DRB1*14:41, DRB1*14:42, DRB1*14:44, DRB1*14:45, DRB1*14:46, DRB1*14:48, DRB1*14:54, DRB1*15:01, DRB1*15:02, DRB1*15:03, DRB1*15:04, DRB1*15:05, DRB1*15:06, DRB1*15:07, DRB1*15:08, DRB1*15:09, DRB1*15:10, DRB1*15:11, DRB1*15:12, DRB1*15:13, DRB1*15:14, DRB1*15:15, DRB1*16:01, DRB1*16:02, DRB1*16:03, DRB1*16:04, DRB1*16:05, DRB1*16:07, DRB1*16:08, DRB3*02:15, DRB4*01:01, DRB4*01:02, DRB4*01:03, DRB4*01:05, DRB4*01:06, DRB4*01:07, DRB5*01:01, DRB5*01:02, DRB5*01:03, DRB5*01:04, DRB5*01:05, DRB5*01:06, DRB5*01:07, DRB5*01:09, DRB5*01:11, DRB5*01:12, DRB5*01:13, DRB5*02:02, DRB5*02:03, DRB5*02:04, DRB5*02:05
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| 566 |
RQ77TV |
77T78V |
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View
Click on a Luminex® HLA allele that shares the eplet RQ77TV to view it at pHLA3D
DQB1*03:01,
DQB1*03:02,
DQB1*03:03,
DQB1*04:01,
DQB1*04:02,
DQB1*06:01,
DQB1*06:02,
DQB1*06:03,
DQB1*06:04,
DQB1*06:09,
DRB1*07:01,
DRB1*09:01,
DRB1*09:02,
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View
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DQB1*03:01, DQB1*03:02, DQB1*03:03, DQB1*04:01, DQB1*04:02, DQB1*06:01, DQB1*06:02, DQB1*06:03, DQB1*06:04, DQB1*06:09, DRB1*07:01, DRB1*09:01, DRB1*09:02
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View (13)
DQB1*03:01, DQB1*03:02, DQB1*03:03, DQB1*04:01, DQB1*04:02, DQB1*06:01, DQB1*06:02, DQB1*06:03, DQB1*06:04, DQB1*06:09, DRB1*07:01, DRB1*09:01, DRB1*09:02
|
| 567 |
RQP37YA |
37Y38A |
|
View
Click on a Luminex® HLA allele that shares the eplet RQP37YA to view it at pHLA3D
DPB1*01:01,
DPB1*11:01,
DPB1*13:01,
DPB1*15:01,
DQB1*03:01,
DQB1*03:02,
DQB1*03:03,
DQB1*04:01,
DQB1*04:02,
DQB1*06:02,
DQB1*06:03,
DQB1*06:04,
DQB1*06:09,
DRB1*10:01,
DRB3*02:01,
DRB3*02:02,
DRB4*01:01,
DRB4*01:03,
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View
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DPB1*01:01, DPB1*11:01, DPB1*13:01, DPB1*15:01, DQB1*03:01, DQB1*03:02, DQB1*03:03, DQB1*04:01, DQB1*04:02, DQB1*06:02, DQB1*06:03, DQB1*06:04, DQB1*06:09, DRB1*10:01, DRB3*02:01, DRB3*02:02, DRB4*01:01, DRB4*01:03
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View (18)
DPB1*01:01, DPB1*11:01, DPB1*13:01, DPB1*15:01, DQB1*03:01, DQB1*03:02, DQB1*03:03, DQB1*04:01, DQB1*04:02, DQB1*06:02, DQB1*06:03, DQB1*06:04, DQB1*06:09, DRB1*10:01, DRB3*02:01, DRB3*02:02, DRB4*01:01, DRB4*01:03
|
| 568 |
RQP57A |
57A |
|
View
Click on a Luminex® HLA allele that shares the eplet RQP57A to view it at pHLA3D
DPB1*01:01,
DPB1*04:01,
DPB1*11:01,
DPB1*13:01,
DPB1*15:01,
DPB1*23:01,
DQB1*02:01,
DQB1*02:02,
DQB1*03:02,
DRB1*14:01,
DRB1*14:04,
DRB1*14:54,
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View
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DPB1*01:01, DPB1*04:01, DPB1*11:01, DPB1*13:01, DPB1*15:01, DPB1*23:01, DQB1*02:01, DQB1*02:02, DQB1*03:02, DRB1*14:01, DRB1*14:04, DRB1*14:54
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View (12)
DPB1*01:01, DPB1*04:01, DPB1*11:01, DPB1*13:01, DPB1*15:01, DPB1*23:01, DQB1*02:01, DQB1*02:02, DQB1*03:02, DRB1*14:01, DRB1*14:04, DRB1*14:54
|
| 569 |
RQP57D |
57D |
|
View
Click on a Luminex® HLA allele that shares the eplet RQP57D to view it at pHLA3D
DPB1*02:01,
DPB1*03:01,
DPB1*04:02,
DPB1*06:01,
DPB1*09:01,
DPB1*10:01,
DPB1*14:01,
DPB1*17:01,
DPB1*18:01,
DPB1*20:01,
DPB1*28:01,
DQB1*03:01,
DQB1*03:03,
DQB1*04:01,
DQB1*04:02,
DQB1*05:03,
DQB1*06:01,
DQB1*06:02,
DQB1*06:03,
DRB1*01:01,
DRB1*01:02,
DRB1*01:03,
DRB1*03:01,
DRB1*03:02,
DRB1*03:03,
DRB1*04:01,
DRB1*04:02,
DRB1*04:03,
DRB1*04:04,
DRB1*09:02,
DRB1*10:01,
DRB1*11:01,
DRB1*11:03,
DRB1*11:04,
DRB1*13:01,
DRB1*13:02,
DRB1*13:05,
DRB1*14:02,
DRB1*14:03,
DRB1*15:01,
DRB1*15:02,
DRB1*15:03,
DRB1*16:01,
DRB1*16:02,
DRB3*02:01,
DRB3*02:02,
DRB4*01:01,
DRB4*01:03,
DRB5*01:01,
DRB5*02:02,
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View
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DPB1*02:01, DPB1*03:01, DPB1*04:02, DPB1*06:01, DPB1*09:01, DPB1*10:01, DPB1*14:01, DPB1*17:01, DPB1*18:01, DPB1*20:01, DPB1*28:01, DQB1*03:01, DQB1*03:03, DQB1*04:01, DQB1*04:02, DQB1*05:03, DQB1*06:01, DQB1*06:02, DQB1*06:03, DRB1*01:01, DRB1*01:02, DRB1*01:03, DRB1*03:01, DRB1*03:02, DRB1*03:03, DRB1*04:01, DRB1*04:02, DRB1*04:03, DRB1*04:04, DRB1*09:02, DRB1*10:01, DRB1*11:01, DRB1*11:03, DRB1*11:04, DRB1*13:01, DRB1*13:02, DRB1*13:05, DRB1*14:02, DRB1*14:03, DRB1*15:01, DRB1*15:02, DRB1*15:03, DRB1*16:01, DRB1*16:02, DRB3*02:01, DRB3*02:02, DRB4*01:01, DRB4*01:03, DRB5*01:01, DRB5*02:02
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View (51)
DPB1*02:01, DPB1*03:01, DPB1*04:02, DPB1*06:01, DPB1*09:01, DPB1*10:01, DPB1*14:01, DPB1*16:01, DPB1*17:01, DPB1*18:01, DPB1*20:01, DPB1*28:01, DQB1*03:01, DQB1*03:03, DQB1*04:01, DQB1*04:02, DQB1*05:03, DQB1*06:01, DQB1*06:02, DQB1*06:03, DRB1*01:01, DRB1*01:02, DRB1*01:03, DRB1*03:01, DRB1*03:02, DRB1*03:03, DRB1*04:01, DRB1*04:02, DRB1*04:03, DRB1*04:04, DRB1*09:02, DRB1*10:01, DRB1*11:01, DRB1*11:03, DRB1*11:04, DRB1*13:01, DRB1*13:02, DRB1*13:05, DRB1*14:02, DRB1*14:03, DRB1*15:01, DRB1*15:02, DRB1*15:03, DRB1*16:01, DRB1*16:02, DRB3*02:01, DRB3*02:02, DRB4*01:01, DRB4*01:03, DRB5*01:01, DRB5*02:02
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| 570 |
RQP67IK |
67I71K |
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View
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View
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DPB1*01:01, DPB1*04:01, DPB1*04:02, DPB1*05:01, DPB1*18:01, DPB1*23:01, DQB1*02:01, DQB1*02:02, DRB1*13:03
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View (9)
DPB1*01:01, DPB1*04:01, DPB1*04:02, DPB1*05:01, DPB1*18:01, DPB1*23:01, DQB1*02:01, DQB1*02:02, DRB1*13:03
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For assistance, please 
